Antibodies

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AF0429 at 1/100 staining human ovarian cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on HuvEc cell lysate using CD147 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0428 at 1/200 staining human Oral squamous cell carcinoma sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
AF0427 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibodWestern blot analysis of extracts from VERO, using ZNF436 Antibody. The lane on the left was treated with blocking peptide.
AF0426 staining NCI-H929  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
AF0425 staining HepG2 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. Samples were then incubated with primary Ab(AF0425 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37¡ãC. An  AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(S0006 1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(S0017 1:600 Green) were used as the secondary antibodWestern blot analysis of ATG4B expression in Mouse brain lysate
Western blot analysis on HeLa cell lysate using TALL-2 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0424 at 1/100 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis of extracts of various samples,using RBAK Antibody.AF0422 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on K562 cell lysate using MMTAG2 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0421 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
AF0418 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibodWestern blot analysis of extracts from Hybridoma cells, using ADNP Antibody. The lane on the left was treated with blocking peptide.
AF0417 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibodWestern blot analysis of extracts from Hybridoma cells, using CIB3 Antibody. The lane on the left was treated with blocking peptide.
Western blot analysis of extracts from Hela, using TINF2 Antibody. Lane 1 was treated with the antigen-specific peptide.AF0415 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis of extracts from 293, using ATRX Antibody.
Western blot analysis on HuvEc cell lysate using TFIP8 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0412 at 1/100 staining human lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis of extracts from various samples, using CBX6 Antibody. Lane 1: 293  treated with  blocking peptide; Lane 2: 293; Lane 3: HepG2; Lane 4: Hela; AF0411 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
AF0410 at 1/100 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondaryAF0410 staining CACO-2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on mouse brain cell lysate using Alpha-synuclein Antibody,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on COS7 cell lysate using CCR7 Antibody.The lane on the left is treated with the antigen-specific peptide.AF0395 staining  A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on HeLa cell lysate using PTPN22 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0391 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
AF0389 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibodWestern blot analysis of extracts from various samples, using NR4A3 Antibody.
 Lane 1: Hela, treated with blocking peptide;
 Lane 2: Hela;
 Lane 3: COS-7.
Western blot analysis of FYCO1 expression in Rat spleen lysate
Western blot analysis of extracts of various samples,using MAN1B1 Antibody.AF0386 staining NIH-3T3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on HeLa cell lysate using ABCF1 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0385 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. Samples were then incubated with primary Ab(AF0385 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37¡ãC. An  AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(S0006 1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(S0017 1:600 Green) were used as the secondary antibod
AF0384 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on mouse liver lysate using RPS9 AntibodyAF0382 staining A549? cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod

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