Antibodies

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Western blot analysis on COS7 cell lysate using FRS2 Antibody,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on HuvEc cell lysate using TTK Antibody,The lane on the left is treated with the antigen-specific peptide.AF0102 at 1/100 staining human Skin tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on Jurkat cell lysate using HSF1 Antibody,The lane on the left is treated with the antigen-specific peptide.
AF0097 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. Samples were then incubated with primary Ab(AF0097 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37¡ãC. An  AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(S0006 1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(S0017 1:600 Green) were used as the secondary antibodWestern blot analysis on mouse brain lysate using 14-3-3 beta/? Antibody.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from 3T3, using eNOS Antibody. The lane on the left was treated with blocking peptide.eNOS Antibody for IHC in human spleen tissue
AF0093 at 1/100 staining human Lymphoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondaryAF0093 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on HuvEc cell lysate using hnRPD Antibody.The lane on the left is treated with the antigen-specific peptide.AF0092 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on COS7 cell lysate using Gz-alpha Antibody.The lane on the left is treated with the antigen-specific peptide.AF0091 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on HepG2 cell lysate using EPHA2/3/4 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0089 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on HeLa cell lysate using Cyclin H Antibody.The lane on the left is treated with the antigen-specific peptide.IHC analysis.
Western blot analysis on COS7 cell lysate using CD226/DNAM-1 Antibody.The lane on the left is treated with the antigen-specific peptide.AF0087 staining hela? cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on HuvEc cell lysate using C/EBP-epsilon Antibody.The lane on the left is treated with the antigen-specific peptide.AF0085 at 1/200 staining human Bone marrow tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on HepG2 cell lysate using Bax Antibody.The lane on the left is treated with the antigen-specific peptide.AF0083 staining C1C12 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on 293 cell lysate using M-CSF Receptor Antibody.The lane on the left is treated with the antigen-specific peptide.This image is a courtesy of anonymous review.
Western blot analysis on Jurkat cell lysate using PECAM-1 Antibody.The lane on the left is treated with the antigen-specific peptide.This image is a courtesy of anonymous review.
Western blot analysis on JK cell lysate using Phospho-VAV3(Tyr173) Antibody,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on HuvEc cell lysate using Phospho-ALOX5(Ser523) Antibody,The lane on the left is treated with the antigen-specific peptide.AF0064 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on HepG2 cell lysate using Phospho-HS1(Tyr378) Antibody,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on K562 cell lysate using Phospho-MAPKAPK5(Thr182) Antibody,The lane on the left is treated with the antigen-specific peptide.AF0061 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on K562 cell lysate using Phospho-MAPKAPK5(Thr182) Antibody,The lane on the left is treated with the antigen-specific peptide.AF0060 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on HT29 cell lysate using Phospho-Gab2(Tyr452) Antibody,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on HepG2 cell lysate using Phospho-EPS15(Tyr849) Antibody,The lane on the left is treated with the antigen-specific peptide.AF0058 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on 293 cell lysate using Phospho-BRCA1(Ser1457) Antibody,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on K562 cell lysate using Phospho-FANCG(Ser383) Antibody,The lane on the left is treated with the antigen-specific peptide.

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