Antibodies

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Western blot analysis on HeLa cell lysate using Phospho-Paxillin(Ser178) Antibody,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on mouse brain cell lysate using Phospho-DYN1(Ser778) Antibody,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on 293 cell lysate using Phospho-RIPK2(Ser176) AntibodyAF0049 at 1/200 staining human lymph node tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on Jurkat cell lysate using Phospho-C-RAF(Ser301) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0047 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on rat heart cell lysate using Phospho-PAK3(Ser154) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0046 at 1/200 staining human Cerebellum tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on Jurkat cell lysate using Phospho-FER(Tyr402) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0044 at 1/200 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on HeLa cell lysate using Phospho-GR(Ser203) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0042 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on HepG2 cell lysate using Phospho-CD227/MUC1(Tyr1229) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0037 at 1/200 staining human colon carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on K562 cell lysate using Phospho-eNOS(Ser615) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0035 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on HuvEc cell lysate using Phospho-hnRPD(Ser83) Antibody,The lane on the left is treated with the antigen-specific peptide.AF0033 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on A549 cell lysate using Phospho-HBP1(Ser402) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0031 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on MOLT cell lysate using Phospho-Retinoblastoma(Thr826) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0030 staining MCF-7? cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on Jurkat cell lysate using Phospho-ERK8(Thr175+Tyr177) Antibody,The lane on the left is treated with the antigen-specific peptide.AF0029 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on HepG2 cell lysate using Phospho-EPHA2/3(Tyr588/596) Antibody,The lane on the left is treated with the antigen-specific peptide.AF0028 staining A549  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on HeLa cell lysate using Phospho-CDC25A(Ser75) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0021 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on mouse brain cell lysate using Phospho-PYK2(Tyr580) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0020 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on Jurkat cell lysate using Phospho-CCR5(Ser349) Antibody.The lane on the left is treated with the antigen-specific peptide.AF0018 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis of Phospho-Akt(Ser473) Antibody expression in Various cells lysates.This image is a courtesy of anonymous review.
Western blot analysis of extracts from mouse skeletal muscle, using RYR2 Antibody.The lane on the right is treated with the antigen-specific peptide.AF0015 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis of extracts from mouse spleen, using CD28 Antibody.The lane on the right is treated with the antigen-specific peptide.AF0014 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
AF0013 at 1/100 staining Human spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondaryAF0013 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis of extracts from HeLa cells, using CAPG Antibody. The lane on the left was treated with blocking peptide.Western blot analysis of extracts from Hela whole cell lysate at 30ug, using CAPG Antibody.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HepG2 cells, using ERLN2 Antibody. The lane on the left was treated with blocking peptide.

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