Antibodies

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Western blot analysis of extracts from rat spleen and HepG2, using SFRS18 Antibody.
Western blot analysis of extracts from mouse brain, using SFRS16 Antibody.
Western blot analysis SFRS12 using COLO205 whole cell lysates
Western blot analysis on K562 cell lysate using ARMX3 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0514 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis on Jurkat cell lysate using ADPGK Antibody,The lane on the left is treated with the antigen-specific peptide.AF0517 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Actin Plant Antibody
Rabbit Anti-Goat IgG (H+L) HRP
Goat Anti-Mouse IgG (H+L) AP
Goat Anti-Rabbit IgG (H+L) AP
Goat Anti-Mouse IgG (H+L) HRP
Goat Anti-Rabbit IgG (H+L) HRP
TUBB3 AntibodyTUBB3 Antibody
MMP-9 Antibody
Cleaved Caspase-7 (Asp198)
Cleaved Caspase-9 (Asp315)  Antibody
Phospho-Gab2 (Tyr643) Antibody
Western blot analysis on HuvEc cell lysate using MEKKK 4 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0512 staining HeLa? cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
AF0511 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibodWestern blot analysis of extracts from Colo205, using ZNF225 Antibody. The lane on the left was treated with blocking peptide.
AF0510 at 1/100 staining human colon cancer tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours AF0510 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis on Jurkat cell lysate using AL2S7 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0509 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis on RAW264.7 cell lysate using VPS72 Antibody,The lane on the left is treated with the antigen-specific peptide.AF0508 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. Samples were then incubated with primary Ab(AF0508 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37¡ãC. An  AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(S0006 1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(S0017 1:600 Green) were used as the secondary antibod
Western blot analysis on HeLa cell lysate using Dyskerin Antibody,The lane on the left is treated with the antigen-specific peptide.AF0507 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod

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