Minute TM Total Protein Extraction Kit for Plant Tissues SD-008/SN-009

Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests)

Manual/protocol

MSDS sheet

Minute™ Total Protein Extraction Kit for Plant Tissues is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract denatured or native proteins from plant tissues (leaves, seeds, soft stems, roots, etc.). This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a specific application. Because protein profiles extracted by these two methods are not identical, one might be superior to the other for various applications. With the use of the protein extraction filter cartridges, total plant-soluble proteins can be extracted from 20-200 mg plant tissue in less than 8 minutes with high protein yield (2-8 mg/ml). Extracted proteins can be used as a good starting material for protein analysis and small-scale protein purification in column chromatography.

Kit includes:

1. 
   
2. Li, P. C., Li, K., Wang, J., Zhao, C. Z., Zhao, S. Z., Hou, L., ... & Wang, X. J. (2019). The AAA-ATPase MIDASIN 1 Functions in Ribosome Biogenesis and Is Essential for Embryo and Root Development. Plant physiology, 180(1), 289-304.
3. Peng, S., Huang, S., Liu, Z., & Feng, H. (2019). Mutation of ACX1, a Jasmonic Acid Biosynthetic Enzyme, Leads to Petal Degeneration in Chinese Cabbage (Brassica campestris ssp. pekinensis). International Journal of Molecular Sciences, 20(9), 2310.
4. Li, X., Li, N., & Xu, F. (2019). Increased autophagy of rice can increase yield and nitrogen use efficiency (NUE). Frontiers in Plant Science, 10, 584.
5. Fu, X., Shi, Z., Jiang, Y., Jiang, L., Qi, M., Xu, T., & Li, T. (2019). A family of auxin conjugate hydrolases from Solanum lycopersicum and analysis of their roles in flower pedicel abscission. BMC Plant Biology, 19(1), 233.
6. Yin, Z., Lu, J., Meng, S., Liu, Y., Mostafa, I., Qi, M., & Li, T. (2019). Exogenous melatonin improves salt tolerance in tomato by regulating photosynthetic electron flux and the ascorbate–glutathione cycle. Journal of Plant Interactions, 14(1), 453-463.
7. Zhen, X., Xu, F., Zhang, W., Li, N., & Li, X. (2019). Overexpression of rice gene OsATG8b confers tolerance to nitrogen starvation and increases yield and nitrogen use efficiency (NUE) in Arabidopsis. PloS one, 14(9), e0223011.
8. Chen, J., Cui, H., Ma, X., Ma, Y., & Li, X. Distribution Differences in the EPSPS Gene in Chromosomes Between Glyphosate-Resistant and-Susceptible Goosegrass (Eleusine indica). Weed Science, 1-28.
9. Wang, C., Chen, S., Dong, Y., Ren, R., Chen, D., & Chen, X. Chloroplastic Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice. New Phytologist.

10.  Chen, J., Huang, H., Wei, S., Cui, H., Li, X., & Zhang, C. (2020). Glyphosate resistance in Eleusine indica: EPSPS overexpression and P106A mutation evolved in the same individuals. Pesticide Biochemistry and Physiology.