![DF12484 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. Samples were then incubated with primary Ab(DF12484 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37¡ãC. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(S0006 1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(S0017 1:600 Green) were used as the secondary antibod](http://www.phscientific.com/cdn/shop/products/15574_thumb_P_1520327928801_{width}x.jpg?v=1592399449)
![DF12484 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. Samples were then incubated with primary Ab(DF12484 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37¡ãC. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(S0006 1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(S0017 1:600 Green) were used as the secondary antibod](http://www.phscientific.com/cdn/shop/products/15574_thumb_P_1520327928801_130x.jpg?v=1592399449)
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