![AF8462 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.](http://www.phscientific.com/cdn/shop/products/10778_thumb_P_1516611793840_{width}x.jpg?v=1592445384)
![AF8462 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.](http://www.phscientific.com/cdn/shop/products/10778_thumb_P_1516611793840_130x.jpg?v=1592445384)
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