![Western blot analysis on 293 cell lysate using Vimentin Antibody.The lane on the left is treated with the antigen-specific peptide.](http://www.phscientific.com/cdn/shop/products/199_thumb_P_1406632369823_{width}x.jpg?v=1591794943)
![AF0292 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary](http://www.phscientific.com/cdn/shop/products/199_thumb_P_1502255681378_{width}x.jpg?v=1591794943)
![AF0292 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod](http://www.phscientific.com/cdn/shop/products/199_thumb_P_1526892869309_{width}x.jpg?v=1591794943)
![Western blot analysis on 293 cell lysate using Vimentin Antibody.The lane on the left is treated with the antigen-specific peptide.](http://www.phscientific.com/cdn/shop/products/199_thumb_P_1406632369823_130x.jpg?v=1591794943)
![AF0292 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary](http://www.phscientific.com/cdn/shop/products/199_thumb_P_1502255681378_130x.jpg?v=1591794943)
![AF0292 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod](http://www.phscientific.com/cdn/shop/products/199_thumb_P_1526892869309_130x.jpg?v=1591794943)
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