Antibodies

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Western blot analysis NUP133 using LOVO whole cell lysates
DF9705 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis NID2 using K562 whole cell lysates
DF9703 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
DF9702 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis NPAS2 using Jurkat whole cell lysates
Western blot analysis GPM6B using Jurkat whole cell lysates
DF9699 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
DF9698 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
DF9697 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis NAV3 using HepG2 whole cell lysates
Western blot analysis NAV2 using HeLa whole cell lysates
Western blot analysis of extracts from mouse brain, using NAV1 Antibody.
Western blot analysis NMB using Jurkat whole cell lysates
Western blot analysis of extracts from HepG2, using NLGN3 Antibody. Lane 1 was treated with the blocking peptide.
Western blot analysis NLGN2 using COS7 whole cell lysates
Western blot analysis NLGN1 using HT29 whole cell lysates
Western blot analysis of extracts from mouse muscle, using NEUROD4 Antibody.
DF9688 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis NCALD using COS7 whole cell lysates
Western blot analysis NBAS using HuvEc whole cell lysates
Western blot analysis NBPF6 using Jurkat whole cell lysates
Western blot analysis NBPF3 using HuvEc whole cell lysates
Western blot analysis NXPH2 using K562 whole cell lysates

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