Antibodies

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Western blot analysis of AURKC using K562 whole  lysates.
DF7669 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22¡ãC. An HRP conjugated goat anti-rabbit antibody was used as the secondary
Western blot analysis of MAP2K5 using K562 whole  lysates.
DF7666 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
DF7664 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod
Western blot analysis of HTT using HuvEc whole  lysates.
Western blot analysis of PPP1R1A using COS7 whole  lysates.
Western blot analysis of CENPA using LOVO whole  lysates.
Western blot analysis of MYCB using K562 whole  lysates.
Western blot analysis of CAD using RAW264.7 whole  lysates
Western blot analysis of IL1R1 using RAW264.7 whole  lysates
DF7656 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis of CYTH3 using COLO205 whole  lysates.
DF7652 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis of extracts from mouse heart, using ARF1 Antibody.
Western blot analysis of ARHGAP5 using HeLa whole  lysates.
DF7646 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod
Western blot analysis of C11orf30 using K562 whole  lysates.
Western blot analysis of RHOQ using COLO205 whole  lysates.
Western blot analysis of CASP2 using K562 whole  lysates.
Western blot analysis of NRAS using mouse liver lysates.
Western blot analysis of CCNA2 using HepG2 whole  lysates.
Western blot analysis of LAMTOR3 using mouse brain lysates.
DF7634 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod

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