BlueRAY Prestained Protein Ladder
The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BlueRAY Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.
-Guide for Molecular Weight Estimation (kDa)
Migration patterns of BlueRAY Prestained Protein Ladder in different electrophoresis conditions are listed below :
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 2~3 μl per well for general Western transferring.
• Apply more for thicker (>1.5 mm) or larger gel.
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
Quality Control :
5 μl of BlueRAY Prestained Protein Ladder (PM006-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
Lane 1: PM019-0500 (Bio-Helix) 5ul
Lane 2: PM008-0500 (Bio-Helix) 5ul
Lane 3: PM007-0500 (Bio-Helix) 5ul
Lane 4: PM006-0500 (Bio-Helix) 5ul
Lane 5: PM001-0500 (Bio-Helix) 5ul
Lane 6: PM019-0500 (Bio-Helix) 5ul
Lane 7: PM001-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul
*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane
We aim to have orders dispatched and delivered to all major areas within 3-5 business days (if in stock in NZ), for regional & rural areas as well as bulk items, this will take 3-10 business days to arrive.
Orders with large quantities or items coming from overseas may take up to 2-3 weeks to be delivered.
Orders are able to be tracked online.
We currently only deliver within New Zealand and do not ship internationally at this stage.
All product prices are displayed in $NZD unless stated otherwise and shipping and GST is additional
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